Figure 2.

Effect of miR-194-3p on the proliferation of primary cultured fibroblasts and HKF cells. (a, b) The effect of miR-194-3p on cell proliferation was detected by MTT assay, and there was a significant difference between groups, ∗∗ P< 0.05. (c) Western blot analysis revealed that the expression of CDK4 was inhibited after the overexpression of miR-194-3p. (d) Real-time PCR showed that the mRNA expression of CDK4 was suppressed after miR-194-3p overexpression, ∗∗ P< 0.05. (e) When miR-194-3p was inhibited, the expression of CDK4 increased. (f) When miR-194-3p was inhibited, mRNA expression of CDK4 was elevated, ∗∗ P< 0.05.