Skip to main content
. 2019 Mar 7;2019:9496419. doi: 10.1155/2019/9496419

Figure 6.

Figure 6

The α3β4-nAChR-related PI3K/AKT signaling pathway is involved in the antiapoptotic effects of a nicotinic agonist in the CVB3-induced apoptosis of NRC. Stimulated by CVB3 for 2 h, NRC were exposed to 25 μM LY294002 and 5 μM α-CTX AuIB for 1 h, respectively, to inhibit PI3K and α3β4-nAChR. 1 μM nicotine was then used to activate nAChRs for 36 h. aP < 0.05 vs. the CVB3-infected group. bP < 0.05 vs. the nicotine-treated group. (a) Photomicrographs (×100) of apoptotic cells. (b) Quantitative analysis of the number of TUNEL-positive cells. (c-e) The expression of pAkt, survivin, and Cleaved Caspase-3 was measured by western blot.