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. 2019 Mar 18;10:545. doi: 10.3389/fmicb.2019.00545

FIGURE 6.

FIGURE 6

Hemolysis and toxicity effect of Dpo48 on mammalian cells. (A) The Dpo48 (7.5 μg/mL) was added to a concentration of 5% (v/v) red blood cells and incubated at 37°C for 1 h with gentle shaking at 60 rpm, and the erythrocytes in PBS and 0.1% Triton X-100 were recorded as negative and positive controls, respectively. The hemoglobin of supernatant was determined at 540 nm. Data are represented as means ±SD (n = 6), and the one-way ANOVA was used to statistical analysis (∗∗∗∗P < 0.0001). (B) HuH-7 (human liver cancer), A549 (human lung carcinoma), and 293T (human embryonic kidney) cell lines were seeded into 96-well microtiter plates (5 × 103 cells/well) and incubated at 37°C for 24 h. The Dpo48 (7.5 μg/mL) or PBS was added to cells and incubated at 37°C for 24 h. The quantification of soluble formazan produced by cellular reduction of MTT was measured at 540 nm. The values are expressed as means ±SD (n = 6), and the independent Student’s t test was adopted in two groups comparison (P > 0.05).