Figure 6.

The capacity of LPS/IFN-γ-stimulated M-MDSC to induce IL-10-producing T cells. (A) A representative analysis is shown of IL-10 and IFN-γ expression within gated CD8⁺ and CD8⁻(CD4⁺) T cell populations after the co-culture with LPS/IFN-γ-stimulated M-MDSC (2 × 10³/well) for 3 days, followed by the IL-2 treatment for additional 3 days. (B) The summarized results from 3 independent experiments are shown as % of IL-10⁺ in CD4⁺ or CD8⁺ T cells co-expressing IFN-γ or not. (C) A representative analysis of IL-10 and FoxP3 expression within CD4⁺IL-4⁺ and CD4⁺IL4⁻ T cells is shown from the experiments performed as in (A). (D) The surface expression of ILT3 and ILT4 were determined by flow cytometry after the staining of LPS/IFN-γ-stimulated M-MDSC and the results are shown as mean MFI or % ± SD of 3 independent experiments. ^*p < 0.05 paired T-test. (E) The summarized data are shown on the % of CD4⁺IL-4⁻FoxP3⁻IL10⁺ (Tr-1) cells ± SD (n = 3) induced in the co-cultures with M-MDSC that were carried out as in (A), either in the presence of anti-ILT-4 Ab or isotype control Ab. ^*p < 0.05 as indicated by the line (RM ANOVA, Tukey post-test).