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. 2019 Mar 18;10:263. doi: 10.3389/fphys.2019.00263

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Copyright © 2019 Séité, Pioche, Ory, Plagnes-Juan, Panserat and Seiliez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Baf A1 treatment affects mRNA levels of gluconeogenic genes. Hepatocytes were treated with DMSO or Baf A1 for 4, 8, 16, or 24 h. Hepatocyte mRNA levels of (A) g6pcb1, (B) g6pcb2, (C) G6pca, (D) fbp1b, (E) pck1, and (F) pck2 were measured using quantitative real time RT-PCR assays. Expression values are normalized with the eukaryotic translation elongation factor 1 α 1 (eef1a1) mRNA. Value are means (n = 6) with standard error represented by vertical bars and were analyzed using two-way ANOVA (P < 0.05), followed by Tukey’s post hoc test for multiple comparisons. When interaction between sampling time and treatment is significant, lowercases letters (a, b, c, and d) represent statistically significant differences (P < 0.05, Tukey’s HSD).