Skip to main content
. 2019 Mar 18;10:263. doi: 10.3389/fphys.2019.00263

Table 1.

Sequences of the primer pairs used in the quantitative real-time RT-PCR assays.

Genes Forward primer Reverse primer
Gluconeogenesis related genes
pck1 ACAGGGTGAGGCAGATGTAGG CTAGTCTGTGGAGGTCTAAGGGC
pck2 ACAATGAGATGATGTGACTGCA TGCTCCATCACCTACAACCT
fbp1b1 CTCTCAAGAACCTCTACAGCCT TCAGTTCTCCCGTTCCCTTC
g6pca GATGGCTTGACGTTCTCCT AGATCCAGGAGAGTCCTCC
g6pcb1 AGGGACAGTTCGAAAATGGAG CCAGAGAGGGAAGAAGATGAAGA
g6pcb2 CCTGCGGAACACCTTCTTTG TCAATTTGTGGCGCTGATGAG
Lipid metabolism related genes
fas TGATCTGAAGGCCCGTGTCA GGGTGACGTTGCCGTGGTAT
plin2 CATGGAGTCAGTTGAAGTCGTC AATTTGTGGCTCCAGCTTGCC
plin3 GATGTCCAACACCGTCACAG TCGATTTCCAACTCGTCCTC
ER stress related genes
chop CTGCACACGGTCTGGAGCTG GGATCTCGTCTGGGATCAGGT
edem1 GAACATCCAAACGGGACAGT TGAGAAGAGGGAGGGAGTCA
xbp1 CAACCCCGAGAACACAGTTT AAGTGACACACGCTGTGGTC
Reference gene
eef1a1 TCCTCTTGGTCGTTTCGCT ACCCGAGGGACATCCTGTG