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. 2019 Mar 18;10:329. doi: 10.3389/fpls.2019.00329

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Copyright © 2019 Lebecque, Lins, Dayan, Fauconnier and Deleu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Upper panels: raw data from ITC experiments. Each peak corresponds to a single injection of 10 μL of a PLPC LUV suspension [5 mM for (A) and (B), 1 mM for (C)] into a solution containing (A) 500 μM nonanoic acid, (B) 75 μM sarmentine and (C) 75 μM sorgoleone at 26°C. LUV suspension and herbicides solutions were buffered at pH 7 with TRIS – HCl. Lower panels: cumulative heats of binding (Σδhi) as a function of the lipid concentration in the cell (C⁰_L). Solid line corresponds to the best fit using the Eq. 4.