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. 2019 Mar 18;10:235. doi: 10.3389/fphys.2019.00235

FIGURE 4.

FIGURE 4

Abnormal Conduction System Structure in Adult MiR-1 TG Mice. (A) Whole mount imaging after fixation and bluo-gal staining of the ventricular conduction system in WT (top) and miR-1 TG (bottom) hearts in the CCS-LacZ background. PF density is reduced in the miR-1 TG adult hearts. (B) Quantitative PCR for genes enriched in PFs demonstrates decreased expression of conduction system genes and either unchanged or only modest changes in core cardiac transcription factors. “∗” denotes p < 0.05, “∗∗” denotes p < 0.01, and “∗∗∗” denotes p < 0.001; NS, non-significant. (C) Averaged ECG tracings derived from limb lead aVF from 8 weeks old WT (top) and miR-1 TG mice (bottom) demonstrated prolonged PR and QRS in the miR1-TG. (D) Comparison of electrocardiographic intervals in adult WT mice (n = 7) and miR-1 TG littermates (n = 8) demonstrate prolonged RR, PR, and QRS intervals in the miR-1 TG mice, reflecting widespread conduction system dysfunction.