Table 3.

Summary of the results for selected immunomarkers in lymph nodes and spleen of striped dolphins (Stenella coeruleoalba), bottlenose dolphins (Tursiops truncatus), and Guiana dolphins (Sotalia guianensis) included in this study.

No	Organ	CAS3	CD3	CD20	CD57	CD68	FoxP3	HLA-DRα	Iba1	IFNγ	IgG	IL4	IL10	Lysozyme	TGFβ	PAX-5
1	LNs	++/3	+/3	+/3	–/0	++/2	+/2	++/3	+/3	+/3	+/3	+/3	+/2	++/3	+/2	+/2
	Spleen	+++/3	+/3	+/3	+/3	++/3	–/0	+,++/3	++/3	+/3	++/3	+/2	++/2	++/3	+/2	+/2
2	LNS	++/2	+/3	+,++/3	–/0	+,++/2	+/2	++,+++/3	+/3	+/3	+/3	+/2	+/2	++/3	+/3	+,++/2
	Spleen	+/2	+/3	+,++/3	+/2	+,++/2	–/0	++/3	++/3	+/2	+/3	+/3	+/2	++/3	+/3	+,++/3
3a	LNs	+/2	+/3	+/3	+/3	++/3	+/2	+,++/3	+/2	++/2	+/3	+/2	+,++/2	+,+++/3	+/2	+/2
	Spleen	+/2	+/2	+/3	–/0	+/2	–/0	+/3	+,++/3	+/2	+/2	+/3	+/2	++/3	+/3	+/2
4	LNs	+++/2	+/3	+/3	+/2	+,++/2	+/3	++,+++/3	+,++/3	+/2	+/2	+/2	+/2	++,+++/3	+/2	+/2
	Spleen	+/2	+/3	+/3	–/0	+,++/2	+/2	++/3	++/3	NE	NE	NE	NE	+,++/3	NE	+/2
5	LNs	+++/3	++/3	+,++/3	–/0	+/2	+/3	++/3	+,++/3	+/2	+,++/3	+,++/3	+,++/2	++/3	+/3	+/3
	Spleen	+/2	+/3	+,++/3	–/0	++/3	+/2	+,++/3	+++/2	+/3	+,++/2	+/2	+,++/2	++,+++/3	+/2	+,++/2
6	LNs	+/2	+/2	+/3	+/3	+/3	+/2	++/3	++/2	+/2	+/2	+/2	+/2	+/3	+/2	+/2
	Spleen	+/2	+/2	+/3	+/2	+/3	+/2	+,++/3	+,++/3	–/0	+/2	+/2	+/2	++/3	+/2	+/3
7	LNs	+/3	+/3	+/3	NE	NE	+/2	NE	+,++/3	NE	NE	+/2	+/2	NE	NE	+/3
	Spleen	+/3	+/2	+/3	NE	NE	+/2	NE	+,++/3	NE	NE	NE	NE	NE	NE	+/3
8	LNs	+/3	+/3	+/3	+/2	++/3	+/2	+,++/3	+/3	+/3	+/3	+/3	+/3	+,++/3	+/2	+/3
	Spleen	++/3	+/3	+/3	+/2	++/3	–/0	++/3	+/3	+/2	+/2	+/3	+/2	++,+++/3	+,++/2	+/3
9	LNs	++/3	++/3	+,++/3	+/3	++/3	+/3	++/3	+/3	NE	+/3	+/3	NE	++,+++/3	NE	+/2
	Spleen	+/2	++/2	+,++/3	+/3	++/2	+/3	+/2	+/3	+/3	+/3	+/2	+/2	++/3	+/2	+,++/2
10	LNs	+++/3	++/3	+,++/3	+/2	++/2	+/3	+,++/3	+/3	+/2	+/3	+/2	+/3	++/3	+/2	+,++/2
	Spleen	+/2	++/3	+/3	+/2	++/3	+/2	+/3	++/2	+/2	+/3	+,++/2	+,++/2	++,+++/3	+/2	+/2
11b	LNs	+/2	+/3	+/3	+/2	+/3	+/2	+/3	+/3	+3	+,++/3	+/3	+/2	++/3	+/2	+/2
	Spleen	+/2	+/3	+/3	–/0	++/3	–/0	+/3	+,++/3	+/3	+,++/3	+/2	+,++/3	+/3	+,++/2	+/2
12	LNs	+/3	++,+++/3	+,++/3	+/3	+,++/2	+/3	++/3	++,+++/3	+/3	++,+++/3	+/3	+/2	+,++/3	+/3	+,++/3
	Spleen	++/2	++/2	++/3	+/3	+,++/3	+/3	++,+++/2	++,+++/3	+,++/3	+,++/3	+/2	+/3	++/3	+/2	+/3
13c	LNs	++/3	+/3	+,++/3	+/3	++/2	+/3	++,+++/3	++,+++/3	+/3	++/3	+/2	+/3	+,++/3	+/2	+/2
	Spleen	++/2	+/3	+/3	+/2	+,++/3	+/3	++/3	++,+++/3	+/3	++/3	+/3	+/3	++/3	+/3	+/2
14	LNs	+++/2	+++/2	++/3	+/2	+,++/2	+/3	++,+++/3	++,+++/3	+/3	+/2	+/2	+/2	+,++/3	+/2	++/2
	Spleen	++/3	++,+++/3	++/3	+/2	+,++/2	+/3	++,+++/3	++,+++/3	+/3	+/3	+/2	+/2	++/3	+/2	++/2
15	LNs	+++/3	+,++/3	+,++/3	+/3	++/2	+/3	++/3	++/3	+,++/3	+,++/3	+/3	+/3	++/2	+,++/2	+,++/2
	Spleen	+++/3	++/2	++/3	+/3	+/2	+/3	++/3	++,+++/3	+,++/3	++/3	+/2	+/3	++/2	+/2	++/2
16	LNs	++/3	+/2	+,++/3	+,++/3	+/3	+/2	+,++/3	++/3	+,++/2	++/2	+/2	+/3	+,++/2	+/3	+,++/2
	Spleen	++/3	++/3	+,++/3	+/3	+,++/2	+/2	+,++/3	++/3	+/3	+/3	+/3	+/2	+/2	+/2	+,++/2
17	LNs	+++/3	++/3	++,+++/3	+/3	+,++/3	+/3	+,++/3	++/3	+/3	+,++/3	+/3	+/3	+,++/3	+/3	+,+++/3
	Spleen	++/3	++/3	+,++/3	–/0	+,++/2	+/3	++/3	++/3	+/3	++/3	+/3	+/3	+,++/3	+/2	+,++/3
18	LNs	++/3	++/3	+,++/3	+/3	+,++/2	+/3	++/3	++/3	+,++/3	+/3	+/3	+/3	++/3	+/2	+,+++/3
	Spleen	++/3	++,+++/3	+,++/2	+/2	+/2	+/3	++/3	++/3	+/3	+,++/3	+/2	+/2	++/2	+/2	+,+++/3
19	LNs	+++/2	+++/2	+,++/3	++/3	++3	+/3	++,+++/3	++,+++/3	+,++/3	+/3	+/3	+,++/3	++,+++/3	+/2	++/2
	Spleen	++/2	++/3	+/2	+,++/3	+,++/3	+/3	++,+++/3	++,+++/3	+/3	+,++/3	+/2	++/2	++/3	+/2	+/2
20	LNs	+/3	+/3	+,++/3	+/2	+,++/2	–/0	+,++/3	++/3	NE	NE	NE	NE	NE	NE	+/2
	Spleen	+/3	++/2	++/3	–/0	+/2	+/3	+,++/3	++/2	+/2	+/2	+/2	+/2	++/3	+/2	+/2
21	LNs	++/3	++/2	++/3	+/2	NE	+/3	+,++/3	++/3	NE	NE	NE	NE	NE	NE	++/2
	Spleen	+++/3	++,+++/2	++,+++/3	+/3	++/3	+/2	+,++/3	+,++/3	+/3	+/3	+,++/2	+/3	++/3	+,++/2	++/2
22	LNs	++/3	+++/3	++,+++/3	+/2	++/2	+/3	++/3	+,++/2	+/2	++,+++/3	+/3	+/2	+ to ++/3	+/2	++,+++/3
	Spleen	++/3	++/3	++,+++/3	+/2	+,++/2	+/3	++/3	+,++/2	++/3	++/3	+/3	+/2	++/3	+/2	++/2
23	LNs	++/2	+++/2	++,+++/3	+/3	+/3	+/2	++/2	++/3		+/2	+/2	+/2	+/3	+/2	++/2
	Spleen	++/2	++/2	++/3	+/2	NE	+/2	+,++/2	++,+++/2	+,++/2	+/2	NE	NE	++/3	NE	++/2
24	LNs	++/2	++/3	++/3	+/3	++,+++/2	+/2	+,++/3	++/3		NE	+,++/2	NE	NE	NE	++/3
	Spleen	++/2	++/3	++/3	+/3	+,++/3	+/3	+,++/3	+++/2	+/3	+,++/3	+/3	+/2	++/3	+/3	++/3
25d	LNs	++/3	++,+++/3	+,++/3	+,++/3	++,+++/2	+/3	++/3	++/2	+,++/2	++/3	++/2	+/3	++/+++/3	+/3	+,++/2
	Spleen	+++/3	++/2	++/3	+/3	++,+++/3	+/3	++/3	++/3	+/2	+/3	+/2	+/3	++/3	+/2	++/2
26	LNs	+/3	+/3	+/3	NE	NE	+/2	++/3	++/3	NE	NE	NE	NE	NE	NE	+/3
	Spleen	+/3	+/3	++/3	NE	NE	+/2	++/3	++/3	NE	NE	NE	NE	NE	NE	+/3
27e	LNs	NE	NE	NE	NE	NE	NE	NE	NE	NE	NE	NE	NE	NE	NE	NE
	Spleen	+/3	+/3	++/3	NE	NE	+/2	++/3	++,+++/3	NE	NE	NE	NE	NE	NE	NE
28	LNs	+/3	++,+++/3	++,+++/3	+/3	–/0	+/3	++,+++/3	++,+++/3	–/0	+/2	+/2	+/2	+/3	–/0	++,+++/2
	Spleen	+/3	++/3	++,+++/3	+/2	+/2	–/0	+,++/3	++/2	–/0	+/2	+/2	+/2	+/2	+/2	++,+++/2
29	LNs	+/3	++,+++/3	++,+++/3	++/2	–/0	+/2	++,+++/3	++/3	+/3	+/2	–/0	+,++/2	+/3	+/2	++,+++/2
	Spleen	+/3	++/3	++,+++/3	+/2	–/0	–/0	+,++/3	++/3	+/3	+/2	+/2	+/2	+/2	+/2	++,+++/2
30	LNs	+/3	++,+++/3	++,+++/3	+/2	+/2	+/3	++/3	++/3	+/3	+/2	+/2	+,++/2	+/3	+/3	++,+++/2
	Spleen	+/3	++/3	++,+++/3	+/2	+/2	+/2	++,+++/3	++/3	+/2	+/2	+/2	+/2	+/2	+/2	++,+++/2

CD, cluster of differentiation; FoxP3, Forkhead Box P3; HLA, human leukocyte antigen (synonym major histocompatibility complex); IFN, interferon; Ig, immunoglobulin; IL, interleukin; TGF, transforming growth factor; Pax-5, paired box 5; NE, not evaluated. Semiquantitative analysis of immunopositive cells: –, no; +, <10%; ++, 10–50%; +++, 50–90%, and ++++, >90% immunopositive cells. Subjective labeling intensity score of immunopositive cells: 0, 1, 2, and 3.

^aMultisystemic hyphate mycosis (lung, kidney).

^bHyphate mycosis (lung).

^cSuspect Brucella coinfection (primarily CNS lesions).

^dSepsis by Staphylococcus aureus (particularly severe CNS and lung lesions).

^eMultisystemic hyphate mycosis (lung, cerebrum).