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. 2018 Dec 10;8(4):366–374. doi: 10.1002/sctm.18-0088

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© 2018 The Authors stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Analysis of commonly listed motivations among papers with new methods or significantly modified methods. (A): Number of articles using metrics to quantify yield of dopaminergic neurons. (B): Percentage of TH+ cells out of total cells. (C): Percentage of TH+ cells out of TUJ1+ cells. (D): The number of articles describing dopaminergic neuron differentiation by progenitor origin marker. (E): Number of control hPSC lines used per study. (F): Temporal length of protocols to differentiate dopaminergic neurons from hPSCs. Frequently cited methods are depicted with colored dots. The five frequently cited methods did not differ significantly from other methods in lines used or length of protocol (p > .05 by t test). Abbreviations: hPSC, human pluripotent stem cells; TH, tyrosine hydroxylase.