Fig. 2.

Btk mediates Osteoclast signaling pathway.

(a, b) Primary human osteoclast precursors were stimulated with M-CSF and RANKL for 48 h. Intracellular phosphorylated Btk (pBtk), total Btk protein (Btk), phosphorylated Lyn (pLyn), total Lyn protein (Lyn), phosphorylated Gab2 (pGab2), total Gab2 protein (Gab2), phosphorylated PLCγ2 (pPLCγ2), total PLCγ2 protein (PLCγ2), phosphorylated BLNK, total BLNK and NFATc1 were detected by Western blot analysis. (b) After immunoprecipitation with an anti-Tec antibody, phosphorylated tyrosine (pTyr) and Tec protein (Tec) were detected by Western blot analysis.