Table-2.

The results are shown as log₁₀ TCID₅₀/ml (mean±SE) of Avian influenza virus inactivating activity by means of reaction in 1%, 5%, and 10% fresh charcoal ash and slaked lime solution in the absence or presence organic materials.

Conditions	Fresh charcoal ash						Slaked lime
	Absence organic materials			Presence organic materialsa			Absence organic materials			Presence organic materials
	1%b	5%	10%	1%	5%	10%	1%	5%	10%	1%	5%	10%
tpcc	7.25±0.50	7.17±0.58	7.42±0.88	7.42±0.29	7.17±0.58	7.42±0.88	7.92±0.52	7.58±0.52	7.58±0.52	7.92±0.52	7.58±0.52	7.58±0.52
0 sd	7.00±0.25	6.75±0.43	7.50±0.90	7.42±0.58	7.17±0.38	7.33±0.72	7.58±0.63	7.17±0.38	7.33±0.72	7.75±0.75	7.42±0.38	7.58±0.63
5 se	NTf	NT	6.33±0.72	NT	NT	6.25±0.66	NT	NT	NT	NT	NT	NT
30 s	NT	NT	3.42±0.88*	NT	NT	4.75±0.43	NT	NT	NT	NT	NT	NT
1 min	NT	NT	NT	NT	NT	3.50±0.87*	NT	NT	NT	NT	NT	NT
5 min	NT	NT	NT	NT	NT	NT	6.25±0.90	5.33±0.63	5.58±0.29	6.83±0.80	NT	NT
10 min	NT	5.42±0.14	NT	NT	NT	NT	4.67±0.29*	3.92±1.01*	3.92±1.13*	6.08±0.38	5.42±0.80	5.42±0.38
30 min	6.67±0.29	4.00±0.50*	NT	NT	5.67±0.72	NT	3.25±0.75*	2.50±0.00*	2.58±0.14*	4.67±0.72*	3.33±0.72*	3.75±0.90*
1 h	6.75±0.25	NT	NT	NT	5.25±1.09	NT	NT	NT	NT	NT	NT	NT
2 h	6.92±0.29	NT	NT	6.92±0.25	3.67±0.63*	NT	NT	NT	NT	NT	NT	NT

^aFetal bovine serum was added to fresh charcoal ash and slaked lime solution as 5% organic materials of total volume.

^bConcentration of fresh charcoal ash and slaked lime solution (%w/v).

^cThe titer converted into an index in log₁₀ of virus control.

^dAdded 1M Tris-HCl before virus.

^eThe titer converted into an index in log₁₀ of the recovered virus after indicated time of treatment such as 5 s, 30 s, 1 min, 5 min, 10 min, 30 min, 1 h, 2 h, 3 h, and 6 h.

^fNT: Not tested.

^*Inactivation effective when RF was ≥3