Table-3.

The results are shown as log₁₀ TCID₅₀/ml (mean±SE) of Newcastle disease virus inactivating activity by the mean of reaction in 1%, 5%, and 10% fresh charcoal ash and slaked lime solution in the absence or presence of organic materials.

Conditions	Fresh charcoal ash						Slaked lime
	Absence of organic materials			Presence of organic materialsa			Absence of organic materials			Presence of organic materials
	1%b	5%	10%	1%	5%	10%	1%	5%	10%	1%	5%	10%
tpcc	8.08±0.38	8.17±0.29	8.08±0.38	8.08±0.38	8.08±0.38	8.08±0.38	8.00±1.09	8.00±1.09	8.00±1.09	8.00±1.09	8.00±1.09	8.00±1.09
0 sd	7.83±0.14	7.92±0.29	7.67±0.76	7.83±0.88	8.08±0.52	8.00±0.50	7.58±0.95	7.67±1.01	7.75±1.32	7.83±0.95	7.58±1.01	7.83±0.95
1 min	NT	NT	NT	NT	NT	NT	7.00±0.50	6.83±0.38	6.92±0.14	NT	NT	NT
3 min	NT	NT	5.25±0.66	NT	NT	6.25±0.43	5.08±0.38	4.75±0.25	4.92±0.38	5.92±0.76	5.92±0.72	5.58±0.76
5 min	NT	NT	4.67±0.29*	NT	NT	5.17±0.14	4.33±0.52*	4.17±0.63*	4.25±1.09*	5.33±0.76	4.42±1.04	4.92±0.58
10 min	NT	NT	4.33±0.29*	NT	NT	4.58±0.14*	NT	NT	NT	3.92±0.80*	4.00±0.90*	3.83±0.95*
30 min	7.17±0.52	6.50±1.39	NT	8.17±0.29	7.50±0.66	NT	NT	NT	NT	NT	NT	NT
1 h	7.25±0.90	5.08±0.58	NT	7.58±0.38	7.00±0.87	NT	NT	NT	NT	NT	NT	NT
2 h	7.17±0.95	3.38±1.15*	NT	7.33±0.72	6.92±0.76	NT	NT	NT	NT	NT	NT	NT

^aFetal bovine serum was added to fresh charcoal ash and slaked lime solution as 5% organic materials of total volume.

^bConcentration of fresh charcoal ash and slaked lime solution (%w/v).

^cThe titer converted into an index in log₁₀ of virus control.

^dAdded 1M Tris-HCl before virus. ^eThe titer converted into an index in log₁₀ of the recovered virus after indicated time of treatment such as 5 sec, 30 sec, 1 min, 5 min, 10 min, 30 min, 1 h, 2 h, 3 h, and 6 h. ^fNT=Not tested.

^*Inactivation effective when RF was ≥3