Table 2.
Sequences of primers used in qRT-PCR.
| Gene | GenBank accession number | Primer sequence | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| Human transcripts | ||||
| ACAN | NM_001113455.2 | F: GAGCCTGAAAACCAGACGGA | 570 | 109 |
| R: TCTCCTCTGTTGCTGTGCTG | ||||
| ADAMTS5 | NM_007038.3 | F: TATGACAAGTGCGGAGTATG | 60.8 | 182 |
| R: TTCAGGGCTAAATAGGCAGT | ||||
| Coll-1 | NM_001003090.1 | F: ACCGACCAAGAAACCACAGG | 61.1 | 226 |
| R: GCACGGAGATTCCTCCAGTT | ||||
| Coll-2 | NM_001006951.1 | F: GACAATCTGGCTCCCAAC | 60.3 | 233 |
| R: ACAGTCTTGCCCCACTTAC | ||||
| GAPDH | NM_001003142.2 | F: GATTGTCAGCAATGCCTCCT | 58.0 | 198 |
| R: GTGGAAGCAGGGATGATGTT | ||||
| Mouse transcripts | ||||
| ACTβ | NM_007393.5 | F: CGACGATGCTCCCCGGGCTGTA | 62.0 | 574 |
| R: CTCTTTGATGTCACGCACGATTTCCCTCT | ||||
| p53 | NM_011640.3 | F: AGTCACAGCACATGACGGAGG | 61.0 | 287 |
| R: GGAGTCTTCCAGTGTGATGATGG | ||||
| TNFα | NM_013693.3 | F: ACAGAAAGCATGATCCGCGA | 62.0 | 295 |
| R: CTTGGTGGTTTGCTACGACG | ||||