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Dental pulp stem cells (DPSC) were plated on tissue culture plastic at a density of 10,000 cells/cm2, stained with Alexa Flour 488 (green) for actin filaments and Propidium Iodide (red) for nucleus and imaged with confocal microscopy after being cultured for (a) 4 days; (b) 6 days; and (c) 6 days with 0.05 mg/mL ZnO nanoparticles added at day 5.
