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. 2017 Jul 11;9(7):277. doi: 10.3390/polym9070277

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of MPTL extracts on the: cellular (A); and nuclear (B) morphology of mouse embryonic fibroblast NIH 3T3 cells. (A) NIH 3T3 cells were exposed to 100% concentrated MPTL extract for the time indicated and examined under inverted microscope using ×20 objective. Apart from healthy, flattened adherent cells and detached round mitotic cells (white arrows), there were only few shrunken cells with blebbing membrane typical for apoptotic cell death (black arrows); (B) NIH 3T3 cells were exposed to 100% concentrated MPTL extract for 72 h and examined under fluorescence microscope using ×40 objective following staining with Hoechst 33342. Shrunken nuclei with intensely stained condensed and pycnotic chromatin were regarded as apoptotic (star). Arrow indicates cell with condensed chromosomes undergoing mitosis.