Table 2.

Zeta potentials of polyplexes prepared at the indicated N/P-ratios.

N/P ratio	(PDMAEMA230)20	B290D240	γ-Fe2O3@silica@-(PDMAEMA540)91	γ-Fe2O3@silica@-(PDMAEMA1037)46
3	−30.7 ± 2.1 mV	0.0 ± 0.1 mV	−9.78 ± 2.41 mV	−8.84 ± 1.29 mV
5	−0.4 ± 4.0 mV	7.3 ± 1.0 mV	−0.24 ± 0.09 mV	−1.99 ± 0.51 mV
7.5	n.d.	n.d.	4.28 ± 0.18 mV	2.95 ± 0.00 mV
10	7.4 ± 1.8 mV	8.8 ± 1.3 mV	5.52 ± 1.01 mV	5.13 ± 0.73 mV
12	n.d.	n.d.	6.73 ± 0.64 mV	6.74 ± 0.32 mV
20	10.1 ± 1.2 mV	10.5 ± 0.6 mV	n.d.	n.d.

(PDMAEMA₂₃₀)₂₀- and B₂₉₀D₂₄₀-based polyplexes were prepared using 15 µg DNA in 1 mL of 150 mM NaCl-solution. γ-Fe₂O₃@silica@(PDMAEMA₅₄₀)₉₁- and γ-Fe₂O₃@silica@(PDMAEMA₁₀₃₇)₄₆-based polyplexes were prepared using 1 µg DNA in 50 µL of 150 mM NaCl-solution followed by dilution in 1 mL Opti-MEM. The zeta potential of the non-complexed DNA is: −26.9 ± 3.2 mV (for 15 µg DNA) and −12.2 ± 1.2 mV (for 1 µg DNA). n.d.: not determined.