Figure 6.

Gene expression levels of the two collagen types, collagen type I (COL-I), and collagen type III (COL-III) in MC3T3-E1 cells exposed to 50 µg/mL of “Na–polyP”, “Mg–polyP-MPs”, “Ca–polyP-MPs” or “col/polyP-MPs”; the controls received no additional component. After the 4-day incubation period the cells were harvested, their RNA was extracted and the steady-state levels of collagen expression were determined by qRT-PCR. The expression levels at time 0 (seeding) and time 4 days are correlated with the one of the housekeeping genes GAPDH. * p < 0.01 (n = 6 parallel experiments; Student’s t-test) with respect to the values of the controls.