Table 4.
Calculation procedure of LD50 using Equation (1) in mice using Karber arithmetic method, 24 h after intra-peritoneal administration.
| Parameters in Equation (1) | HA/PVA 2c | HA/PVA2c-MTX (D2Y) | HA/PVA23-MTX (D3WY) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Doses (mg/kbw) | 4000 | 3000 | 2500 | 2000 | 1500 | 1000 | 500 | 250 | 2000 | 1500 | 1000 | 500 |
| Number. of mice/group | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| Deaths | 4 | 3 | 0 | 4 | 3 | 3 | 1 | 0 | 4 | 3 | 1 | 0 |
| A | 1000 | 500 | 500 | 500 | 500 | 250 | 500 | 500 | 500 | |||
| B | 3.5 | 1.5 | 3.5 | 3 | 2 | 0.5 | 3.5 | 2 | 0.5 | 3.5 | ||
| a × b | 3500 | 750 | 1750 | 1500 | 1000 | 125 | 1750 | 1000 | 250 | |||
| LD50, mg/kbw | 2937.5 | 906.25 | 1250 | |||||||||