Figure 4.

In (A,B) cellular association of polyplexes with N2a cells after 30 min incubation at 4 °C as determined by flow cytometry is plotted; In (C,D) cellular internalization of polyplexes after 45 min incubation at 37 °C followed by removal of extracellularly-bound polyplexes is displayed; Logarithmic X-scale in (A–D) represents Cy5 fluorescence of polyplexes; (E) Luciferase reporter gene expression in N2a cells after 0.75 h (without pattern) and after 24 h (with pattern). Transfections were performed at two different ratios: N/P = 6 (red) and N/P = 12 (green). (F) Cell viability assay was performed in parallel.