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. 2017 Apr 19;9(4):142. doi: 10.3390/polym9040142

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Luciferase reporter gene expression in human prostate cancer cell line DU145 as well as in (B) human hepatocellular cancer cells Huh7 after 0.75 h (without pattern), and after 24 h (with pattern). Transfections were performed at two different ratios: N/P = 6 (red) and N/P = 12 (green); (C) Luciferase gene expression at 48 h after intratumoral administration of pCMVLuc polyplexes at N/P = 12 into Huh7 tumor-bearing mice. Luciferase gene expression is presented as relative light units per gram tumor (RLU/g tumor; n = 5, mean ± SEM). Lysis buffer RLU values were subtracted.

(A) Luciferase reporter gene expression in human prostate cancer cell line DU145 as well as in (B) human hepatocellular cancer cells Huh7 after 0.75 h (without pattern), and after 24 h (with pattern). Transfections were performed at two different ratios: N/P = 6 (red) and N/P = 12 (green); (C) Luciferase gene expression at 48 h after intratumoral administration of pCMVLuc polyplexes at N/P = 12 into Huh7 tumor-bearing mice. Luciferase gene expression is presented as relative light units per gram tumor (RLU/g tumor; n = 5, mean ± SEM). Lysis buffer RLU values were subtracted.