Table 2.
Particle size (Z-average), polydispersity index (PDI), and zeta potential of plasmid DNA (pDNA) polyplexes formed in HEPES-buffered 5% glucose (HBG) buffer determined with dynamic laser-light scattering (DLS). Mean of three measurements of the same sample is indicated. Polymer at nitrogen-to-phosphate ratio (N/P) 12 and 2 µg plasmid DNA (pDNA) were separately diluted with HBG pH 7.4 to 30 µL each. Then solutions were mixed and incubated for 30 min. Polyplexes were diluted to 800 µL with 10 mM NaCl pH 7.4 prior to measurement. Particle size displayed as number mean can be found in Table S2. # indicates internal library compound IDs.
| Polymer # | Abbreviation | Average size (nm) | Average PDI | Average zeta potential (mV) |
|---|---|---|---|---|
| 689 | 3-arm | 126.8 ± 2.7 | 0.15 ± 0.02 | 32.0 ± 3.5 |
| 1088 | PEG12 | 97.9 ± 1.1 | 0.14 ± 0.02 | 6.7 ± 2.3 |
| 1091 | PEG24 | 111.6 ± 0.9 | 0.16 ± 0.01 | 3.1 ± 0.6 |
| 1120 | PEG48 | 87.2 ± 1.4 | 0.33 ± 0.05 | 1.6 ± 0.5 |
| 1094 | PAS4 | 127.7 ± 0.8 | 0.12 ± 0.01 | 7.1 ± 1.5 |
| 1097 | PAS8 | 147.0 ± 1.8 | 0.15 ± 0.01 | 3.4 ± 0.8 |