Table 1.

Results of the primary antibody and OVA adsorption.

Surface IgG a	Primary (μg/cm2)	IgG Coverage b (%)		OVA a (μg/cm2)	OVA Coverage c (%)		Ratio of Molecules d (OVA/IgG)
		End-on	Side-on		End-on	Side-on
PS	0.17 ± 0.01	9	63	0.72 ± 0.01	171	266	14.1
(PDDA/PAA)3	0.10 ± 0.01	5	36	0.21 ± 0.01	50	77	7.0
(PDDA/PAA)3PDDA	0.40 ± 0.01	21	146	1.91 ± 0.1	455	707	15.9

^a The dimension was calculated from the apparent area of filter (the diameter was 13 mm) (n = 3). ^b The dimensions of the IgG are 14.5 nm × 8.5 nm × 4 nm [33]. When one assumes full monolayer coverage, the amount of IgG adsorption was 1.85 and 0.27 μg/cm² in the end-on and side-on positions, respectively. ^c The dimensions of the OVA are 7 nm × 5 nm × 4.5 nm [34]. When one assumes full monolayer coverage, the amount of OVA adsorption was 0.42 and 0.27 μg/cm² in the end-on and side-on positions, respectively. ^d The molecular weights of IgG and OVA were 150 and 45 kDa, respectively.