Table 2.

Amount of the primary antibody immobilization on the (PDDA/PAA)₃.

Concentration of antibody (μg/mL)	Primary IgG a (μg/cm2)	IgG coverage b (%)
		End-on	Side-on
10	0.28 ± 0.01	15	104
20	0.42 ± 0.01	23	156
30	0.56 ± 0.03	30	207

^a The dimension was calculated from the apparent area of filter (diameter was 13 mm). (n = 3); ^b The dimensions of the IgG are 14.5 nm × 8.5 nm × 4 nm [33]. When one assumes full monolayer coverage, the amount of IgG adsorption was 1.85 and 0.27 μg/cm² in the end-on and side-on positions, respectively.