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Confocal images of multi-layered printing of fibroblast and keratinocytes (FB and KC) on the cell culture dish. (A) Z stacking using immunofluorescent images showed interlayer distance ~75 μm was observed. (B,C) keratin-containing KC layer and β-tublin-containing KC and FB. Bright-field images on (D) KC layer and (E) FB layer also confirmed the immunohistochemistry findings. Reproduced from [77]. Copyright 2009 with permission from Elsevier.
