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Double-staining performed in 21 days skin graft with keratin-14 red) and involucrin (green) stained the lowermost and uppermost layers respectively. Some evidence of co-localization (yellow to orange) in the intermediate layers was found. Both stains were found to be partially retained in Ker-CT-Ras keratinocytes, but were far less in Ker-CT-Ras-p53 keratinocytes. Scale bar: 40 µm. Reprinted under open access distribution license from [100].
