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. 2016 Mar 17;8(3):83. doi: 10.3390/polym8030083

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Structure difference in lipid molecule-decorated NPs and its effect on endocytosis. (A) Schematic of the two-stage-microfluidic platform; (B) Cryo-TEM images of monolayer shell lipid-polymer hybrid nanoparticles (MP P-L NPs) and bilayer shell lipid-polymer hybrid NPs (BP P-W-L NPs), the electron density showed that the BPs had a lipid bilayer. While the MPs have only one layer of lipids; (C) Plot of the fluorescence emission spectrum of BPs and MPs. It suggests that the BPs contain more water; (D) Plot of the Young’s moduli for MPs and BPs. MPs had higher Young’s modulus than the BPs with same size of 40 nm in diameter; E: The HeLa cells had a higher uptake of MPs than BPs. Chlorpromazine (CPZ), Ethylisopropyl amiloride (EIPA), and Genistein are used for inhibiting clathrin-mediated endocytosis, macropinocytosis, and caveolae-mediated endocytosis, respectively. The figures are adapted from References [50,51] with permission.