Fig. 5. (A) GluC digestion sites (dashed lines) and 1O2 oxidation sites (arrows) identified for LASD(HHCC). (B) HPLC chromatograms obtained before (bottom) and after 1 min (middle) and 5 min (top) irradiation of a solution of Zn·LASD(HHCC) (60 μM) containing rose bengal (2 μM), in 20 mM Pi (D2O pD 7.0). The species identified by digestion and ESI-MS analysis of each collected peak are displayed. (C) CD spectra before (solid line) and after 10 min irradiation (dotted line) of a solution of Zn·LASD(HHCC) (30 μM) containing rose bengal (3 μM), in 20 mM Pi (H2O pH 7.0).
