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. Author manuscript; available in PMC: 2019 Mar 25.
Published in final edited form as: Prostate. 2016 Feb 2;76(8):703–714. doi: 10.1002/pros.23162

Fig. 3.

Fig. 3.

In vitro characterization of Ala-Pro-PABC-Emetine analog 10. (A) Conversion of compound 10 (200 μM) to emetine in the presence of serum free media (SFM), purified DPPIV (30 nM; activity ≥4,500units/μg protein), 10% fetal bovine serum (FBS), purified recombinant FAP (300 nM). (B) Inhibition of hydrolysis to free emetine by FAP inhibitor Ac-Gly-boroPro (200 μM) in 10% FBS. (C) Inhibition of growth of PrEC cell growing in FAP/DPPIV negative serum free media over 1–3 days exposure to emetine or 10 at 100 nM.