Figure 3. Decreased number of PV+INs in hippocampal sections of HI injured mice at p18.

Representative photomicrographs of PV IR of hippocampus from sham (A, D), NT (B, E) and TH (C, F) treated male (A₁ to F₁) and female (A₂ to F₂) mice at p11 (A-C) and p18 (D-F) with details (A’-F’, magnification bar = 10μ). Number of PV+INs per mm² in the hippocampus of male (G₁) and female (G₂) sham (white boxes; n (males) = 5 at p11 and 6 at p18; n (females) = 3 at p11 and 4 at p18), NT (black boxes; n (males) = 5 at p11 and 9 at p18; n (females) = 3 at p11 and 5 at p18), and TH (grey boxes; n (males) = 5 at p11 and 9 at p18; n (females) = 5 at p11 and 5 at p18) treated mice at p11 and p18. Data represented using box and whisker plots to show the PV+ INs/ mm² counts in whole hippocampus (HIP), and in CA1, CA3 and DG regions in separate at p11 and p18. Boxes are limited by the 75^th and 25^th percentiles (interquartile range, IQR) and whiskers are limited by the last data point within 1.5 times the IQR from the median (continuous line inside the box).*, p<0.05. (n=3–9 mice per treatment, time and sex). Representative high magnification photomicrographs showing morphology of PV + INs of sham (H), NT (I), and TH (J) at p18. Fold change in PV mRNA levels in forebrain of male and female mice (K) treated with NT (black) and TH (grey) vs. sham (discontinuous line sitting at 1)*, p<0.05. (n=5 mice per treatment and sex).