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. Author manuscript; available in PMC: 2019 Mar 25.
Published in final edited form as: Methods Mol Biol. 2016;1400:117–130. doi: 10.1007/978-1-4939-3372-3_8

Fig. 3.

Fig. 3

Quantitative transposition and homologous recombination assays under G418 and nourseothricin selection. Transposition levels (a) and homologous recombination levels (b) in cells containing the wild-type Tf1 plasmids with either G418 or Nat selection are compared to levels in cells containing an INfs Tf1 expression vector. Transposition levels are reduced roughly 20-fold in the INfs controls (a) and homologous recombination levels are reduced roughly twofold in INfs controls when compared to WT (b). All samples are normalized to WT-Tf1 transposition or recombination levels. Each strain was assayed in triplicate (n = 3) and the results averaged