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. Author manuscript; available in PMC: 2019 Mar 25.
Published in final edited form as: Methods Mol Biol. 2019;1869:207–230. doi: 10.1007/978-1-4939-8805-1_18

Fig. 6. Targeting 2: Inserting Distal Element.

Fig. 6.

(a) Targeting 2 cassette fragment isolated with HindIII and SacII to facilitate gap repair homologous recombination. (b) Final H3f3a knock-in targeting vector generated by successful insertion of targeting 2 Neo selection/distal element cassette (2.3 kb) into H3f3a intron 3/exon 4. EagI sites mark diagnostic digest points to verify final gene targeting vector fidelity.