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. 2019 Mar 11;146(5):dev168559. doi: 10.1242/dev.168559

Fig. 3.

Fig. 3.

ROP2, ROP4 and ROP6 are required for root hair formation and elongation. (A) Representative images of roots of wild type Col-0 (WT) and the following ROP mutants: rop2, rop4i-3, rop6, rop2 rop4i-8, rop2 rop6, rop4i-5 rop6 and rop2 rop4i-4 rop6. Scale bar: 1 mm. (B) Measure of root hair length (in mm) in all genotypes mentioned above. More than 150 root hairs from at least ten growing roots for each genotype and each replicate (three replicates) were measured (n=450). (C) Hair density measurement (number of hairs per mm) for all genotypes mentioned above, n=30 roots per genotype (10 for each biological replicate). Data are mean±s.d. analysed using Student's t-test (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001). (D-F) Measure of the area (D), length (E) and number of existing patches (F) on a 480 µm region of wild-type and rop2 rop4 rop6 roots, just after the meristem. Data are average±s.d. (n=30 roots for each genotype) analysed using Kolmogorov–Smirnov test (**P<0.01, *P<0.05). See Fig. S3B for representative images.