Fig. 5.

Depletion of PML increases sensitivity to cytolytic virus. (A,B) The three virus-resistant ALT cell lines (A) and the HT1080 (TEL) cell line (B) were treated with siNC or siPML (and/or siATRX for HT1080) for 72 h, and depletion of PML was confirmed by western blot. Graphs show PML NB number (top) and coefficient of resistance to viral infection (bottom); mean±s.e.m., n=3 independent experiments; *P<0.05, **P<0.01 using a paired two-tailed t-test. (C) Coefficient of resistance versus PML NB numbers was plotted for untreated and PML and/or ATRX-depleted (siPML/siATRX) cells, with the three resistant ALT lines and HT1080 cells indicated in colored circles; the remaining data points are as previously shown in Fig. 2D.