Fig. 2.

Podoplanin-dependent MSC migration is mediated through Rac-1. Podoplanin-negative (PDPN−) and -positive (PDPN+) MSCs seeded onto an 8 µm pore filter were treated with inhibitors against (A) RhoA–RhoC (CT04), (B) ROCK (Y27632) or (C) Rac-1 (NSC23766) over a range of concentrations. Migration was assessed at 48 h by counting the number of cells detached from the upper and lower chamber. Data are expressed as the number of cells in the lower chamber as a percentage of the total cell count for both chambers. In all cases, one-way ANOVA: P<0.01; *P<0.05, **P<0.01 by Dunnett’s post-test compared to untreated control for each MSC type. Data are mean±s.e.m. from n=4 independent experiments using different biological donors for each cell type in each independent experiment.