Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 25;12:66. doi: 10.1186/s13068-019-1401-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 2 — a Percent of mutants obtained with various RNP-based HDR. b Gel electrophoresis image of the WT, NR-KO and mixed colonies upon colony PCR. c Phenotypic characterization of NR mutants, the NR mutants were observed to bleach in media with nitrate as the sole nitrogen source while they grew similar to WT in media with ammonia as the nitrogen source (The re-streak plates for characterization included in Additional file 1: Data S13). d Sequencing result of a NR-KO mutant showing precise integration of the Zeocin resistance cassette into the host genome knocking out the NR gene