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. 2019 Mar 25;15:5. doi: 10.1186/s12993-019-0156-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Participation of the DRN in MCS-induced antinociception. Quantification of Egr-1 (A) and 5HT (B) IR in the DRN of naive (without surgical intervention), sham (with epidural electrodes but without stimulation) and stimulated (MCS, 1.0 V/60 Hz/210 µs, 15 min) rats. Values represent the mean ± SEM (n = 5 animals per group). *p < 0.05 and **p < 0.01 in comparison to the naive group, after Bonferroni’s post hoc test. Photomicrographs illustrating the 5HT-IR in the DRN of sham (C) and stimulated (D) rats. 5HT serotonin, DRN dorsal raphe nucleus, IR immunoreactivity, MCS motor cortex stimulation. Scale bars: 200 µm