Table 2.
The t1/2 and Tm values (50% loss of activity) of SaDAE wild-type and mutants
SaDAEase | t1/2 (h) | Tm (°C) |
---|---|---|
Wild type | 2.04 | 67.0 |
S209C | 3.25 | 70.0 |
S188D/M190F | 3.71 | 71.5 |
S209C/S188D/M190F | 4.57 | 74.2 |
V105A/S209C/S188D/M190F | 4.68 | 74.6 |
The t1/2 and Tm values (50% loss of activity) of SaDAE wild-type and mutants
SaDAEase | t1/2 (h) | Tm (°C) |
---|---|---|
Wild type | 2.04 | 67.0 |
S209C | 3.25 | 70.0 |
S188D/M190F | 3.71 | 71.5 |
S209C/S188D/M190F | 4.57 | 74.2 |
V105A/S209C/S188D/M190F | 4.68 | 74.6 |