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. 2019 Mar 25;7:48. doi: 10.1186/s40478-019-0702-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Ablation of retinal microglia following PLX5622 treatment. Retinal flat mounts from uninfected tgGFP/RFP mice without drug treatment (a) and with PLX5622 treatment (b). a In untreated mice, GFP-expressing microglia span the entire retina and appear highly branched with fine delicate processes (inset). b After 7 days of PLX5622 treatment, microglia have disappeared from the entire retina. Scale bars = 200 μm for a and b, 20 μm for inset