Fig. 5.

Immunohistochemical staining of tgGFP/RFP retinas using anti-Iba1, anti-GFP and anti-RFP antibodies. a anti-Iba1 staining of uninfected retina shows absence of microglia in the ONL, IS, and OS, one Iba1-positive cell (red) is visible in the OPL (arrow). b In response to scrapie infection of retina, there is significant migration of Iba1-positive cells (arrow) to the ONL, IS and OS. c anti-GFP staining (brown), indicative of Cx3cr1 expression in microglial cells, is not present in the ONL, IS and OS of the uninfected retina. d In the infected retina, microglia expressing GFP (brown) are present at a similar density to Iba1-positive cells in (b). e anti-RFP staining (brown), specific for Ccr2-expressing monocytes is not visible in the uninfected retina. f A rare RFP-positive cell (arrow) is present in the IS of a scrapie-infected retina. Note the two unstained nuclei (arrowhead), which are likely Iba1/GFP positive cells. Scale bar is 25 μm