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. Author manuscript; available in PMC: 2019 Mar 25.
Published in final edited form as: J Immunol. 2015 Aug 26;195(7):3293–3300. doi: 10.4049/jimmunol.1500800

Figure 2.

Figure 2.

L-ornithine synthesis from L-arginine or 13C5 L-citrulline. (A) Schematic of normal (12C5) L-ornithine synthesis from L-arginine (right) and heavy (13C5) L-ornithine synthesis from L-citrulline (left). Enzymes are depicted in parentheses. (B-E) PDMs, cultured in media containing L-arginine and heavy 13C5 isotope L-citrulline (1.0 mM (B, C) or 0.2 mM (D, E)), were infected with Mb BCG (moi = 5) with and without IFN-y stimulation for 48 hours. Uninfected PDMs (–) were also cultured for 48 hours. Supernatants (B, D) and cell lysates (C, E) were analyzed by LC-MS/MS to detect native (12C5) and heavy (13C5) L-ornithine (n = 4). Data are combined from 2 experiments. Error bars, SD.