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. Author manuscript; available in PMC: 2019 Mar 25.
Published in final edited form as: J Immunol. 2015 Aug 26;195(7):3293–3300. doi: 10.4049/jimmunol.1500800

Figure 3.

Figure 3.

Differential NO and L-ornithine production in mycobacteria-infected MΦs cultured in L-arginine or L-citrulline. (A-F) Mycobacteria-infected PDMs with and without IFN-γ stimulation were cultured in L-arginine, L-citrulline, or both AAs at 1.0 mM (A, C, E) or 0.2 mM (B, D, F). (A, B) NO production was determined by analyzing supernatant nitrite (NO2-) amounts by Griess assay (n = 8). (C, D) Total L-ornithine combining intracellular and extracellular amounts. (E, F) The ratio of NO2- to L-ornithine was determined by dividing the concentration of NO2- by L-ornithine (intracellular plus extracellular). Data are presented as fold change compared to MΦs cultured in L-arginine media (n = 4). Data are combined from 2 experiments. Error bars, SD. * p < 0.05, *** p < 0.001 by Student’s t test.