Figure 5.

NO production and mycobacterial control in MΦs cultured in supraphysiological L-arginine and L-citrulline. (A-C) PDMs (n ≥ 5) were left alone or pre-stimulated with IL-4 + IL- 10 for 24 hours. Following PBS wash, PDMs were cultured in R-free C-DMEM containing 1.0 mM L-arginine [R] or 1.0 mM L-citrulline [CIT] and infected with Mb BCG plus IFN-γ, with and without BEC for 72 hours. At 72 hours post-infection, NO was determined by Griess assay.(B).Data are the mean NO₂^- amounts. Mb BCG CFU were determined from lysed MΦs (C). Data are the individual CFU. Black lines represent the mean. Data are combined from 2 experiments. Error bars, SD. * p < 0.05, ** p < 0.01 by Student’s t test. ns, not statistically significant.