Figure 2.

Streptococcus pneumoniae membrane composition. Lipid analysis was performed during the exponential growth phase (deep coloring), early and late stationary phase (middle and light coloring, respectively). (A) Relative amount of lipids by polar head groups; GDG, glucosyldiacylglycerol; GGDG, galactosylglucosyldiacylglycerol; PG, phosphatidylglycerol; and CL, cardiolipin. (B) Relative amount of acyl chains, saturated in green, unsatured in red. The inset show the total relative amount of saturated acyl chain. Error bars are the standard deviation of three independent experiments. (C) Relative amount of fully saturated lipids. (D) Surface pressure isotherms of monolayers prepared with lipid extract from exponentially growing cells recorded at 30°C (cyan), 37°C, (blue), and 43°C (magenta).