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. 2019 Mar 18;10:351. doi: 10.3389/fmicb.2019.00351

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Copyright © 2019 Calvez, Jouhet, Vié, Durmort and Zapun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of lipids in S. pneumoniae depends on the cell shape. (A) Cells were labeled simultaneously with DOPE-rho (red) and DMPE-NBD (green) prior to further growth for 60 min. (B) The cell wall of cells labeled and chased as in (A) was digested by peptidoglycan hydrolases. (C) Growing cells expressing FtsZ-mKate (yellow) labeled with fluorescent lipid II-NBD (cyan) and the corresponding demographs. (D) The cell wall of fixed cells labeled with lipid II-NBD was digested by peptidoglycan hydrolases.