Figure 6.

Quantification of zearalenone and its metabolites in pregnant wild-type and Bcrp^−/− mice. A, Western blot of placental homogenates from pregnant wild-type and Bcrp^−/− mice analyzing BCRP protein expression (72 kDa) on gestation day 14. GAPDH (37 kDa) was used as a loading control. B–D, Concentration of zearalenone and its metabolites detected in serum (B), fetuses (C), and matched placentas (D) 1 h after tail vein injection as determined by LC-MS. Total concentration was determined after incubation of samples with β-glucuronidase overnight at 37°C. Data represent the mean ± SD (n = 4–5). Asterisks (*) represent statistically significant differences (p < .05) compared with wild-type mice.