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. 2019 Mar 22;7:e6626. doi: 10.7717/peerj.6626

Figure 2. Comparing RT-qPCR and RNA-Seq results from independent experiments to validate combining the RNA-Seq datasets.

Figure 2

(A–D) are the diurnal patterns of expression of MtFKF1 (Medtr8g105590), MtCDF1 (Medtr2g059540), MtCDF2 (Medtr5g041420 and MtCDF4 (Medtr6g012450) respectively measured over a diurnal time course using RT-qPCR. The first four hours which overlap with the RNA-Seq data are shaded in grey. Error bars are standard errors of two biological replicates. Samples consist of two fully expanded trifoliate leaves with two biological replicates per time point. For MtFKF1 these samples were pooled and error bars are standard errors of technical replicates (using Medtr7g089120 as a reference gene) while for MtCDF1, MtCDF2 and MtCDF4 these are standard errors of biological replicates (using PP2A as a reference gene). (E–H) are the corresponding transcript abundances from the RNA-Seq datasets. The points represent TPM values of the individual replicate libraries plotted with a LOESS smoothed line of best fit for both SD (blue and dotted) and LD (orange and line) samples.