Figure 4. Contrasting timepoint specific expression profiles of the genes which alter their pattern of gene expression in response to the change of photoperiod conditions and clustering the relative changes in expression over time.

(A) contrasts the 3,912 DE genes (>2-fold difference and >10 mean normalised reads) by the timepoints at which they are DE and plots their overlap. The principal chart plots the size of the overlaps and the supplementary chart presents the number of genes DE (both up or down) in LD relative to SD for each timepoint. (B) Heatmap of standardised gene abundances (such that the average expression value is zero and the standard deviation is one) of all 9,516 genes which alter their pattern of gene expression grouped into 18 clusters using c-means clustering. The number of genes in each cluster is listed alongside. (C) Mean standardised abundances for selected clusters. Specifically cluster 3 was selected as it contains MtFTb1 which characteristically peaks at ZT4 in LD (orange and line) with no expression in SD (blue and dotted). Clusters 9, 11 and 17 were selected as their pattern is opposite to that of cluster 3 in that they peak at ZT4 in SD. Mean standardised abundances for all clusters are plotted in Fig. S5.