Figure 2.

H19 promotes HTR-8 adhesion by inhibiting miRNA let-7. (A) Examples of the trophoblast spheroid and adhesion assays with HTR-8 are shown. Spheroid formation occurs under optimal conditions for the HTR-8 cells. A total of 1000 cells are seeded into an ultra-low attachment 96-well plate with a lid flat bottom in media supplemented with 5% serum and 2 mg/mL methylcellulose aggregated into compact spheroids within 24 h. Then, the trophoblast spheroids are transferred into a 6-well plate filled with HESCs, and the attachment ratio is calculated after 12 h of co-culture. The attachment ratios in the (B) H19-knockdown group, (C) H19-overexpression group, and (D) combination of H19 knockdown and let-7 inhibitor group are shown.