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. 2019 Feb 26;157(5):475–484. doi: 10.1530/REP-18-0466

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VIP activated primordial follicle through ERK-mTOR signalling way. Ovaries were cultured with 10⁻⁷ mol/L VIP + 10⁻⁸ mol/L U0126 or 8.75 × 10⁻⁸ mol/L rapamycin for 3 days. (A and B) Western blot was conducted to investigate the level of protein phosphorylation in the ERK-mTOR signalling pathway, as well as AKT and FOXO3A. Values are mean ± s.d. of at least three experiments. (*P < 0.05 compared with the VIP group. ^& P < 0.05 compared with the VIP + U0126 group. ^# P < 0.05 compared with the VIP + Rapa group).